Ultrafiltration

The holy grail in ultrafiltration (UF) is to eliminate sample fouling and to enhance recovery and purity of target protein during the concentration or buffer exchange step. Generon has introduced a 2.5 ml non-stick UF concentrator to provide the highest possible protein recoveries. This is due to 2 factors: the low protein binding CTA membrane and critically, the design of the X-spinner which ensures that ultrafiltration is in opposite direction to the centrifugal force. The contra design also ensures that the filter does not clog. This is the first centrifugal concentrator designed with membrane proteins as a key application.

Proteus X-spinner 2.5 Features:

• Membrane Type - Cellulose Triacetate and Polyethersulphone
• MWCO - 5 kDa, 10 kDa, 20 kDa, 100 kDa & 300 kDa MWCO
• Sample Volume Range - 0.1- 2.5 ml
• Hold-up Volume - 25 ul
• Pack Sizes - 24 pc & 96 pc
• % Protein Recoveries - >99%
• Max g force - 2,500 g (Swing Bucket Rotor, 2,000 g (Fixed Angle Rotor)

Protein Ark also offer ultrafiltration membrane disks for concentrating, buffer exchange and de-salting all proteins, peptides and other macromolecules in all sizes of Amicon stirred cells. Available in a broad range of diameters and MWCO’s in either Reconstituted Cellulose (RC) or proprietary-treated Polyethersulfone (PES) to ensure low non-specific protein binding.

   Why is it so good?

• 2.5 ml non-stick UF concentrator to provide the highest possible protein recoveries.
• Unique design prevents filter blocking 
• Highest protein recoveries available- Guaranteed
• Viscous samples:-serum, proteins stored in 10-15% glycerol
• Dilute protein solutions:-why risk losing your protein?
• Sticky and hydrophobic proteins such as fibronectin.
• Protein fractions post Size Exclusion or IMAC
• De-proteinization of blood and serum samples
• Separating fee analyte or label from bound analytes/bound label

Ultrafiltration Membrane Discs Features:

• Optional membrane selection - Polyethersulfone (Ultralow-protein binding) - Regenerated Cellulose (high resistance)
• Disc diameter - 25, 44.5, 62, 76 and 90 mm
• Wide MWCO range - 5 to 100 kDa
• Broad pH range - pH 3-12 (RC) / pH 1-14 (PES)
• High operating pressure - 75 psi (5 bar) max