Proteus HiFliQ FPLC Columns

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Protein Ark HiFliQ columns pre-packed and ready to use with pre-charged Nickel-NTA and Cobalt-NTA Agarose resin for affinity purification of poly-histidine tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Available in 1 ml and 5 ml HiFliQ column sizes with high binding capacity and minimal ion leakage. Compatible with all common chromatography HPLC and FPLC instruments (including ÄKTA™ FPLC’s), and low pressure pumps and syringes using an appropriate adaptor.

Specifications:

• Column volume: 1 or 5 ml resin

• Column construction: Polypropylene

• Resin: Super Ni-NTA Agarose

and Super Co-NTA Agarose

• Base Matrix: 7.5% cross-linked Agarose

• Ni-NTA capacity: 50-75 mg (per 1 ml resin)

Co-NTA capacity: 40-50 mg (per 1 ml resin)

• Flow rate: 1 ml/min (1 ml), 1-5 ml/min (5 ml)

• Max pressure: 0.5 MPa (72 psi)

• Universal 10.32 (1/16’’) UNF threads:

Inlet Female/Outlet Male